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KMID : 1098420170250060389
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Korean Journal of Medicinal Crop Science
2017 Volume.25 No. 6 p.389 ~ p.396
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Genetic Diversity Analysis of Wood-cultivated Ginseng using Simple Sequence Repeat Markers
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Gil Jin-Su
Um Yu-Rry
Byun Jae-Kyung
Chung Jong-Wook
Lee Yi
Chung Chan-Moon
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Abstract
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Background: Panax ginseng C. A. Meyer is wood-cultivated ginseng (WCG) in Korea which depends on an artificial forest growth method. To produce this type of ginseng, various P. ginseng cultivars can be used. To obtain a WCG similar to wild ginseng (WG), this method is usually performed in a mountain using seeds or seedlings of cultivated ginseng (CG) and WG. Recently, the WCG industry is suffering a problem in that Panax notoginseng (Burk.) F. H. Chen or Panax quinquefolium L. are being sold as WCG Korean market; These morphological similarities have created confusion among customers.
Methods and Results: WCG samples were collected from five areas in Korea. After polymerase chain reaction (PCR) amplification using the primer pair labeled with fluorescence dye (FAM, NED, PET, or VIC), fragment analysis were performed. PCR products were separated by capillary electrophoresis with an ABI 3730 DNA analyzer. From the results, WCG cultivated in Korea showed very diverse genetic background.
Conclusions: In this study, we tried to develop a method to discriminate between WCG, P. notoginseng or P. quinquefolium using simple sequence repeat (SSR) markers. Furthermore, we analyzed the genetic diversity of WCG collected from five cultivation areas in Korea.
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KEYWORD
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Panax ginseng, Simple Sequence Repeat, Wood-Cultivated Ginseng
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